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differentiation thp 1 monocytes  (ATCC)


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    ATCC differentiation thp 1 monocytes
    Differentiation Thp 1 Monocytes, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 20870 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/differentiation thp 1 monocytes/product/ATCC
    Average 99 stars, based on 20870 article reviews
    differentiation thp 1 monocytes - by Bioz Stars, 2026-02
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    Uptake of DiI-LDL by PMA differentiated <t>THP-1</t> monocytes. Representative false-color images of fluorescent LDL (DiI-LDL) uptake by macrophages. Cell nuclei are shown in red, and DiI-LDL is shown in green. A, DiI-LDL only. B, DiI-LDL with 1 μm apo(a). C, DiI-LDL with 1 μm apo(a) + 6 mm TX. D, dose-response effect of TX on the apo(a)-induced uptake of DiI-LDL. Results from a representative experiment with S.D. within the experiment are shown. Fluorescence of DiI-LDL within the fixated cells was quantified by imaging using Arrayscan VTI and the software Bioapplication. The scale bar (white line) is 100 μm.
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    Image Search Results


    Uptake of DiI-LDL by PMA differentiated THP-1 monocytes. Representative false-color images of fluorescent LDL (DiI-LDL) uptake by macrophages. Cell nuclei are shown in red, and DiI-LDL is shown in green. A, DiI-LDL only. B, DiI-LDL with 1 μm apo(a). C, DiI-LDL with 1 μm apo(a) + 6 mm TX. D, dose-response effect of TX on the apo(a)-induced uptake of DiI-LDL. Results from a representative experiment with S.D. within the experiment are shown. Fluorescence of DiI-LDL within the fixated cells was quantified by imaging using Arrayscan VTI and the software Bioapplication. The scale bar (white line) is 100 μm.

    Journal: The Journal of Biological Chemistry

    Article Title: Identification and analyses of inhibitors targeting apolipoprotein(a) kringle domains KIV-7, KIV-10, and KV provide insight into kringle domain function

    doi: 10.1074/jbc.RA119.011251

    Figure Lengend Snippet: Uptake of DiI-LDL by PMA differentiated THP-1 monocytes. Representative false-color images of fluorescent LDL (DiI-LDL) uptake by macrophages. Cell nuclei are shown in red, and DiI-LDL is shown in green. A, DiI-LDL only. B, DiI-LDL with 1 μm apo(a). C, DiI-LDL with 1 μm apo(a) + 6 mm TX. D, dose-response effect of TX on the apo(a)-induced uptake of DiI-LDL. Results from a representative experiment with S.D. within the experiment are shown. Fluorescence of DiI-LDL within the fixated cells was quantified by imaging using Arrayscan VTI and the software Bioapplication. The scale bar (white line) is 100 μm.

    Article Snippet: LDL uptake by phorbol 12-myristate 13-acetate (PMA)-differentiated THP-1 monocytes Differentiation of THP-1 monocytes (ATCC–TIB-202) into macrophages was done in RPMI 1640 medium with Glutamax + 10% FCS (Invitrogen) and 50 ng/ml PMA (Sigma) by diluting the cells and seeding the 30,000–40,000 cells/well in 100 μl/well into Packard View plates followed by incubation for 4 days at 37 °C and 5% CO 2 .

    Techniques: Fluorescence, Imaging, Software